From the n-hexane extract of the leaves of Markhamia stipulata var. Canaense V.S. Dang, using colunm chromatography, two flavonoids, one anthraquinone and one triterpenoid were isolated. Based on the analysis of high resolution mass spectroscopy and nuclear magnetic resonance as well as comparison with published data, their structures were identified as 4'.7-O-dimethylapigenin (1), narigenin (2), tectoquinone (3), mollic acid (4). Compound 1 and 4 were isolated from this plant for the first time.