Article info.

ABSTRACT

Received 28 Dec 2017
Revised 04 Jun 2018

Accepted 30 Nov 2018

Recently, extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli was isolated from cultured striped catfish, red tilapia and wild fish in the Mekong Delta, Vietnam. ESBL genes are located on plasmids, facilitating their spreads among Gram negative bacilli bacterial species. To better understand the dissemination of resistance genes in aquatic system, the antimicrobial susceptibility patterns and the molecular characteristics of ESBL-producing E. coli isolates were investigated using disk diffusion method and polymerase chain reaction. The results indicated that the proportion of antibiotic resistance of ESBL-producing E. coli was relatively high in most types of antibiotics except meropenem and cefoxitin. Considerably, multiple drugs resistance was recorded at high percentage, including 100% for ESBL-producing E. coli isolates of snakehead fish, 90% depended on the figure for striped catfish, 85% for ESBL-producing E. coli isolates of red tilapia, and 50% for that of wild fish. Besides, the number of ESBL-producing E. coli isolates carrying multiple ESBL genes were 90%, significantly higher than those of carrying single ESBL gene at just 10%. The B2 virulence group was mainly isolated from wild fish, which was higher compared to groups of culture fish. Moreover, the majority of isolates harbored multiple sulfonamides resistance genes (72.2%), which was significant higher compared to the percentages of isolates carrying single gene (27.8%). The study illustrated that there were the significant widespread of antibiotic resistant genes of ESBL-producing E. coli as well as a considerable ratio of multidrug resistance.

Keywords

ESBL-producing Escherichia coli, extended-spectrum beta-lactamase genes, fish, the Mekong Delta, sul genes

Cited as: Ly, T.H., Tuyet Hoa, T.T.T. and Huyen, H.M., 2018. Antibiotic resistance and molecular characteristics of extended-spectrum beta-lactamase-producing Escherichia coli isolated from fish pond. Can Tho University Journal of Science. 54(8): 114-123.