Galactose biosensor based on ZnO nanorods were fabricated and modified through the addition of Au nanoparticles. The sol-gel method was utilized to grow ZnO nanorods on indium tin oxide-coated glass substrates (FTO), and the Au nanoparticles were modified on ZnO nanorods by hydrothermal method. Well aligned hexagonal structured ZnO nanorods with a diameter from 40 nm to 60 nm were obtained. The Au nanoparticles were spheres with diameters varied from 6 nm to 8 nm. After these Au NPs were attached to ZnO nanorods electrode, galactose oxidase enzyme (GOx) was immobilized on this electrode in 3.5 hours to form the working electrode in galactose biosensor (GOx|Au-ZnO|FTO). The cyclic voltammetry method was used to test the activity of the working electrode in galactose solution 200 mM concentration. The cyclic voltammetry result showed that the current intensity of the GOx|ZnO|FTO electrode in galactose solution is about 0.09 µA/mm2. On the other hand, with the GOx|Au-ZnO|FTO electrode, this value increases to 0.2 µA/mm2.
Keywords
Au nanoparticle, cyclic voltammetry method, galactose biosensor, ZnO nanorod
Cited as: Ha, L.P.P., Huy, H.D., Tra, L.N.M. and Trung, T.Q., 2018. The properties of ZnO nanorods modified by Au nanoparticles for galactose biosensor application. Can Tho University Journal of Science. 54(5): 81-87.
Tạp chí khoa học Trường Đại học Cần Thơ
Lầu 4, Nhà Điều Hành, Khu II, đường 3/2, P. Xuân Khánh, Q. Ninh Kiều, TP. Cần Thơ
Điện thoại: (0292) 3 872 157; Email: tapchidhct@ctu.edu.vn
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