The results of survey different methods for isolation bacteriophages and evaluation of their effect in controlling bacterial leaf blight disease on rice caused by Xanthomonas oryzae pv.oryzae (Xoo) was conducted in laboratory and nethouse conditions. By the direct method and enrichment method, there were 107 bacteriophages isolated from 62 strains Xoo original from 6 provinces of Mekong Delta (i.e An Giang, Dong Thap, Kien Giang, Can Tho, Hau Giang, Soc Trang). Phages with coded ΦXaVL12, ΦXaDT63c, ΦXaHG48b, ΦXaDT60b, ΦXaAG68a showed higher the parasitic efficiency to many Xoo strains, and bacterial strain XaAG73 was parasitized by almost isolated bacteriophages strains. By comparision about lytic ability of 5 bacteriophages on bacterial strain XaAG73, phage ΦXaDT60b showed the highest lytic ability to bacterial lawn with the biggest diameter of plaque in different time course observation. In the nethouse, four phages ΦXaVL12, ΦXaDT63c, ΦXaDT60b, ΦXaAG68a showed effect in reduction of bacterial leaf blight infection and last 15 days. Among them, phage ΦXaDT60b exposed the most effect in disease reduction by spraying phage suspention (10⁸ pfu/ml) on the leaf suface before and after pathogen inoculation.