Measurement of anti-rabies antibodies in dog antiserum samples collected from slaughter-houses in CanTho city was conducted from February 2015 to November 2015 by using indirect enzyme-linked immunosorbent assays (ELISA) (SERELISA^® Rabies Ab Mono Indirect kit, France). A total of 184 dog antiserum samples collected from slaughter-houses at 6 districts located in CanTho city were tested for anti-rabies antibodies. The results revealed14.13% of antiserum samples containing protective antibodies against rabies viruses. The percentage of antisera containing protective antibodies in more than one-year-old dogs was significantly higher than below one-year-old dogs (17.51% in 1-2.5 year-old and 31.25% in >2.5 year-old vs 8.5% in