Termitomycesmushrooms are affluent in bioactive components contributing to their therapeutic properties. This study aimed to investigate the antioxidant, antibacterial, antifungal activities and the toxicity of Termitomycesextracts. Termitomycesmushrooms were collected from the South of Vietnam, separated into different parts, air-dried, ground into powder, and extracted with methanol and ethanol at a divergent temperature of evaporation. Termitomycesmycelial biomass extract was discovered to be efficient in scavenging the free radicals through DPPH assay. The extract exhibited potent efficacy against Gram-negative (Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhimurium) and Gram-positive (Bacillus cereus, Staphylococcus aureus) bacteria along with Candida albicansfungus applying the disc-diffusion method. Termitomycesextract underwent in vitro and in vivoexperiments revealed no toxicity. The Termitomycesmycelial biomass extract is a potential source in developing novel antioxidant, antibacterial and antifungal agents.