This study was conducted to evaluate the effect of dietary VP28-Bacillus subtilis (Bacillus subtilis displaying VP28 on the spore surface) on the survival and innate immune response of black tiger shrimp (Penaeus monodon). The shrimp were fed with pellets containing B. subtilis spores displaying VP28 under CotB-VP28, CotB-GST-VP28 at three different feeding regimes, followed by a challenge experiment with white spot syndrome virus (WSSV). The effectiveness of the spores were assessed by: (i) the number of immunological parameters containing total hemocyte count, phenoloxidase activity, and superoxide dismutase activity, and (ii) the survival rate of shrimps after being challenged with WSSV. The results showed that: (i) continuous feeding regime of B. subtilis spores displaying VP28 had a higher survival rate; (ii) total haemocyte count (2,29x10⁴ cell/mm³; 2,17x10⁴ cell/mm³), PO activity (0,334; 0,316) and SOD activity (0,844; 0,831) of shrimp fed by feed containing CotB-VP28, CotB-GST-VP28 were significantly higher than control group (pB. subtilis spores displaying VP28 (CotB-VP28) in the protection of shrimp against WSSV.