Phalaenopsis orchids known to all as kind of commercial flowers which are commonly called the moth orchid belonging to the family Orchidaceae. The present study was conducted to determination of total phenolic compounds, flavonoid content, and antioxidant activity of ethanol extracts of Phalaenopsis hybrids. Total phenolic content of extract was estimated by Folin-Ciocalteau reagent assay in methanol extract. Content of total phenolics was highly found to be 8.55 ± 0.02 mg GAE/g DW. Antioxidant activity of root and leaf extracts was evaluated by DPPH free radical scavenging assay, reducing power method and ß-carotene bleaching method. The antioxidant activity of root extracts was higher those of leaf. An increase in absorbance at 700nm revealed reducing power of the extract. There are eleven phenolic compounds were identified include protocatechuic acid, p-hydroxyl benzoic, vanillic acid, caffeic acid, syringic acid, vanillin, ferulic acid, sinapic acid, p-coumaric acid, benzoic acid and ellagic acid. Ferulic acid, p-coumaric acid and sinapic acid are concentrated largely in the root samples. The result suggests that the root extracts from Phalaenopsis hybrids could use as a source of antioxidants.