Article info.

ABSTRACT

Received 14 Jan 2019
Revised 06 Jun 2019

Accepted 30 Jul 2019

Fucoidan, a polysaccharide composed of L-fucose with sulfate ester, has a wide range of pharmacological uses due to its massive number of biological activities. Besides, brown seaweed, fucoidan is also found from Kappaphycus alvarezii alga in the central coast of Vietnam. In the study, the influences of fucoidan extraction from Kappaphycus alvarezii alga by using HCl was assessed. The response surface methodology was applied to optimize three factors of material-to-solvent ratio, temperature, and time for the extraction. The monosaccharide composition was determined by using high-performance liquid chromatography. The fucoidan structures were studied using Fourier transform infrared (FT-IR) and ¹³C-NMR (nuclear magnetic resonance). Antioxidant activity was determined using DPPH method. Monosaccharides of fucoidan were determined by high-performance liquid chromatography. The optimal results were HCl 1.39M at 85.1°C in 3.64 hours; fucoidan content was 47.9 µg/mL. The FT-IR spectrum of fucoidan contains specific peaks of sulfate group in fucoidan in the axial or equatorial position. ¹³C-NMR spectrum and monosaccharides of fucoidan were specific characteristic properties.

Keywords

Fucoidan, HCl, Kappaphycus alvarezii, optimizing, RMS

Cited as: Nhon, H.T.N., Huyen, D.T. and Thinh, N.V.N., 2019. Study on the optimal conditions for fucoidan extraction from Kappaphycus alvarezii. Can Tho University Journal of Science. 11(2): 22-30.