Study was conducted in to assess effects of probiotics supplement isolated from different sources on water quality, growth and survival rate of white shrimp Litopenaeus vannamei larvae. Experiment consisted of four treatments with three replications (1) control treatment without probiotic supplement and three other treatments were supplemented probiotics (2) Lactobacillus sp. isolated from the sauce of macerated fish (3) Enterococcus hirae isolated from sauce of macerated shrimps and (4) lactic acid bacteria Pediococcus sp. isolated from the grape wine. Larvae of white leg shrimp were reared in the 7 m³ cement tanks at density of 300 larvae L^-1 and at salinity of 30 g L^-1. Bacteria were supplemented every three days at a rate of 10⁶ CFU g^-1 during larval rearing. Results showed that addition of probiotic bacteria in the culture tanks helped to improve water quality as well as survival and growth of L. vannamei larvae were significantly higher (pLactobacillus sp. gave better results compared to other probiotic bacteria.