The aim of this study is to isolate the bacterial strains which degrade Chlorpyrifos from rice-upland crop soils in the Mekong Delta. Twelve soil samples were taken at Cai Lay-Tien Giang, Cho Moi-An Giang and Binh Tan-Vinh Long. Soil bacteria were enriched in mineral salt medium solution containing 20 mg/L of Chlorpyrifos as the only carbon source for bacterial growth. The results showed that one bacterial community was enriched and degraded Chlorpyrifos. One bacterial strain (coded as BT_C8.9) that was isolated from this community degraded 41.07 % of Chlorpyrifos after 30 culture days. According to the sequencing of 16S-rRNA gene, this bacterial strains was identified as Microbacterium sp. C8.9