Application of biofertiliser and saving irrigation water are two important techniques to sustain rice production in the Mekong Delta. The objective of this study is to quantify the impact of BioGro fertilizer and alternating wetting and drying irrigation technique (AWD) on grain yield and methane and nitrous oxide emission in a rice crop. Trials were conducted in two consecutive rice crops. A two-factors experiment was laid out in a split-split plot where the main-plots were  water management practices: alternate wetting and drying irrigation or continuous flooding as farmer?s practice. BioGro and N fertiliser were applied to sub plots at 3 levels: (1) 90 kg N/ha (100% treatment), (2) BioGro + 45 kg N/ha and, (3) 45 kg N/ha. Experiment 2 was designed as similar to experiment 1 except without level (3) of N fertilizer. This experiment was used for methane and nitrous oxide gas measurement. In the Summer-Autumn crop, grain yield of plots received BioGro was 5 tones/ha, equivalent to those received 100% of N fertilizer. The AWD method reduced 400m³ /ha, about 22% of irrigation water inputs and increased grain yield (170 kg/ha) compared to continuously flooding practice. In Autumn-Winter crop, the application of BioGro contributed to the reduction of methane and nitrous oxide emission compared with farmer?s conventional method. Water saving irrigation practice helped to reduce methane gas emission but increased nitrous oxide emission. Results showed that BioGro biofertiliser application and AWD practice are feasible and economical for intensive rice production system in the Mekong Delta.