Black garlic (aged garlic) supplementation in diet has been shown to be beneficial to patients. Recently, its nano-size and pharmacological role in the prevention and treatment of cancer has received increasing attention. The present study was designed to use cancer cells, harmful microbes, and enzymes cause diabetes and Alzheimer's disease as test models to evaluate their effects. The obtained data from the assay indicated that black garlic nanoparticles system are positive for oral cancer cells, liver cancer cells, lung cancer cells, monkey kidney cancer cells, skin cancer cells and negative for breast cancer cells. Black garlic did not show activity against Staphylococcus aureus, Enterococus faecium, E. coli and Candida albicans at concentrations less than 256 µg/mL while black garlic nano-particles system showed inhibited activity on seven microorganism lines in this study. Black garlic nanoparticles might inhibit -glucosidase enzyme which is involved diabetic but not AChE that is involved Alzheimer's.