Changes in land use/land cover (LULC) have a significant impact on the changes in ecosystem service values. This study aims to assess the impacts of LULC changes on provisioning ecosystem services (pES) in the Long Xuyen Quadrangle region (LXQ) from 2000 to 2021.  Using Landsat 5 TM and Landsat 8 OLI imagery, this study analyzes the normalized difference vegetation index (NDVI) time series using MODIS imagery to monitor and evaluate the impacts of LULC changes in the LXQ region. Additionally, interviews with farmers were conducted to estimate the ecosystem service values of different rice cultivation models. The research results in the creation of LULC maps for the LXQ in 2000 and 2021, with relatively high reliability, including overall accuracy (OA) of 83.18% (K = 0.772) and 94.26% (K = 0.9284), respectively. The main LULC change observed in the LXQ region from 2000 to 2021 is the conversion from wetlands to double-cropped rice and triple-cropped rice systems. The study identified an area of 54,667.722 ha (26.24%) where wetlands were converted to double-cropped rice, and an area of 36,779.633 ha (17.65%) where wetlands were converted to triple-cropped rice. Furthermore, the estimated ecosystem service values after the conversion from wetlands to triple-cropped rice were approximately 122,504,931 VND/ha/year, and for double-cropped rice, it was approximately 87,772,604.88 VND/ha/year. The research findings indicate that wetland agriculturalization, including double-cropped rice and triple-cropped rice, has affected the ecosystem service provision in the LXQ region. These research results provide significant considerations for future comprehensive valuation of ecosystem service values in the LXQ, Vietnamese MD.