The study aims to assess the quality of soil and water environment in Phu My Species and Habitat Conservation Area, Kien Giang province. Water samples were collected at 16 sites in 7 different habitats (Lepironia articulata - Eleocharis dulcis habitat, Lepironia articulata - Melaleuca cajuputi habitat, Lepironia articulata - Ischaemum rugosum habitat, Eleocharis dulcis habitat, Lepironia articulata - Eleocharis ochrostachys habitat, Eleocharis ochrostachys habitat, Rice field habitat) and four canals in the area for analysing pH, dissolved oxygen (DO), chemical oxygen demand (COD), total nitrogen (TN), total phosphorus (TP), aluminum (Al³⁺) and iron (Fe²⁺). Water level, velocity and flow discharge in the canals were also measured. Soil was sampled at 15 sites in different habitats for analysing pH, conductivity (EC), organic matter (OM), TN, TP, available phosphorus (P₂O₅), available potassium (K₂O), and Al³⁺. Results showed that water quality of the study area had low pH, relatively high concentration of Al3+ and low nutrient (TP). Saline water intruded into canals inside the conservation area. The soil was characterized as acid sulfate soil since pH was low and acidity and exchangeable Al³⁺ were high. The concentration of TP, P₂O₅, TN and K₂O in soil ranged from low to medium whereas OM level was high. Canals in the conservation area are unevenly distributed. The average flows and velocities varied between locations, but the levels of water changed slightly. Low water exchange may lead to water pollution in the area. The study provides useful information on soil and water properties for sustainable management and development in Phu My Species and Habitat Conservation Area.