This study aimed to evaluate antioxidant and antibacterial activities of Euphorbia hirta L. extract. The results showed that the extract of Euphorbia hirta L. displayed good antioxidant activities using DPPH, ABTS•+ methods. In addition, this extract gave moderate antibacterial effect against three bacterial strains including Aeromonas dhakensis, Aeromonas hydrophila and Vibrio parahaemolyticus causing diseases on aquaculture species. Total phenolic and total flavonoid contents were 140.85±4.72 mg GAE/g extract and 202.42±10.25 mg QE/g extract, respectively. These results indicated that Euphorbia hirta L. is a potentially medicinal plant containing lots of natural antioxidant and antibacterial compounds.