An experiment was conducted to determine the effect of diet supplementing with tributyrin (TBU) or probiotic (PRO) in diet on egg performance and quality of laying hens from 20-30 weeks age. A total of 2160 Hisex Brown laying hens at the 20 weeks of age were randomly distributed in a completely randomized design experiment, with 3 treatments and 3 replicates, each replicate consisted of a line with 60 pens (4 birds/pen). The experimental data was collected during 10 weeks. Treatments used: (1) Control (Cont): Basal diet (B) without any supplementation; (2) TBU: B + Tributyrin; (3) PRO: B + Probiotic, all supplements were supplied at 0.5 g/kg feed in the diets. The results showed that, average daily feed intake was not affected by tributyrin or probiotic supplementation in the diets (P>0.05). But a little improvement hen day production, egg weight and feed conversion ratio in TBU and PRO compared to Cont group. Tributyrin supplementation significantly increased eggshell thickness (PE. colicontent in feces were decreased in TBU and PRO supplementation diets compared with control group. The conclusion of this experiment is adding tributyrin or probiotic at 0.5 g/kg feed could lightly improve hen day production, feed conversion  ratio, increased eggshell thickness and reduce E. coli in feces.

Keywords: Hen- day production, Hisex Brown, laying hen, Tributyrin, Probiotic.