Pasteurella multocida (P. multocida) is one of the predominant pathogens that mostly cause respiratory diseases in domestic animals, such as goats. To determine P. multocida serotypes and the prevalence of pathogenic and antibiotic-resistance genes the PCR method was used. A total of 143 isolated P. multocida strains were collected from 289 healthy hybrid Boer-Saanen goats’ nasal samples in the Mekong Delta, Vietnam, from March to June 2023. A total of 143 P. multocida strains, serotype B accounted for the highest proportion (51.05%), followed by serotype A (14.69%), and the lowest was serotype E (0.70%) while (39.86%) of strains could not be determined serotypes. Among the six virulence genes surveyed, the sodA gene (56.64%) had the highest presence, while the ompH gene (4.20%) had the lowest presence. Pathogenic genes were present mainly in serotypes A and B; tbpA was frequently detected in serotype A (66.67%), and sodA was commonly detected in serotype B (56.16%). There were 14 virulence gene combinations in 59/109 (54.13%) serotyped P. multocida strains, and the pattern of sodA + toxA + tbpA was prevalent at the highest rate (12.84%). Moreover, among the eight investigated antibiotic resistance genes, the sulII gene had the highest presence rate (74.13%), compared to the tetA gene with the lowest presence rate (13.29%). Gene sulII was mainly detected on strains belonging to serotypes A (80.95%), B (83.56%), and F (77.78%). A total of (77.98%) of serotyped P. multocida strains indicated multi-harbor from two to six antibiotic-resistance genes, and the most common pattern was aadB + sulII (10.09%). The prevalence of five pathogenic P. multocida serotypes harboring diverse antibiotic-resistance genes isolated from nasal samples could be a critical issue in treating and preventing the respiratory diseases caused by P. multocida in goats in the Mekong Delta.