In the recent years, culture of snakehead fish in the lined tanks has been rather rapidly developed in the Vietnamese Mekong Delta. According to previous researches, more than 23% of households culturing snakehead fish in lined tanks and 90% interviewed households did not treat wastewater before discharging into receiving water bodies. In this study, treatment of wastewater from the lined tank of snakehead fish was performed by combining the coagulation-flocculation, sedimentation and cold plasma technologies. Wastewater was treated at lab-scale condition. Study results showed that the effluent treatment efficiency on the model reached over 70% for BOD, COD, phosphorus and total coliforms. Quality of wastewater after treatment met the Vietnamese national regulation for industrial wastewater quality (QCVN 40:2011/BTNMT). However, electrical conductivity of water after treatment increased and the treatment efficiency of cold plasma was not high; it is necessary to be improved in further researches.