Article info.

ABSTRACT

Received date: 09/12/2015

Accepted date: 30/11/2016

Aromatic rice has become popular owing to its aroma. Growing demand for aromatic rice has spurred interest of breeders. In this study, twenty five BC₁F₁ rice lines of backcross hybrid combinations were used to evaluate aromatic characteristics through sensory test and using microsatellite markers. Twelve lines containing aroma and one without aroma were chosen through sensory test.  Allele Specific Amplification with four primers (External Antisense Primer, External Sense Primer, Internal Non-fragrant Sense Primer, and Internal Fragrant Antisense Primer) were used for identifying fgr gene locus in 25 rice lines. Analysis of PCR results showed that BC₁F₁ population segregated into 1:1 ratio (homozygous fragrant: heterozygous non-fragrant). Thus, twelve promising lines were identified with homozygous fragrant genotype.

Keywords

Aromatic rice, microsatellite markers, sensory test

Cited as: Chi, N.T.Y. and Ngon, T.T., 2016. Screening of aromatic rice lines by using molecular marker and sensory test. Can Tho University Journal of Science. Vol 4: 100-104.