A phytochemical study on petroleum ether-diethyl ether (v/v 1:1) extract led to the isolation of one sterol with unsual side chain, 22-dehydro-24-isopropylcholesterol (1) and one common triterpenoid oleanolic acid (2). Compound 1 has been previously identified as a marine invertebrates sterol, here we report the first time its appearance in terrestrial source Polyscias fruticosa. Their structures, including absolute configuration, are elucidated unambiguously by X-ray diffraction, spectroscopic data and comparison with literature.