Researching folk literature in relation to folklore has been used effectively by folklore experts in the world. Recently, in Vietnam, this research direction has also been becoming more popular and gaining practical values ​​in finding the material and spiritual cultural values, as well as behavioral cultural-values with the natural environment which are hidden in folk literature, including folk-songs. Folk-songs in the Mekong Delta are spiritual product of people living in the land with interlacing rivers and canals. It is the characteristic that contributes to creating specific features for folk-songs of a new land. By applying some methods like statistics, analysis, synthesis, and comparison, this article presents some features of river culture in the folk-songs of the Mekong Delta to distinguishing them from Vietnamese folk-songs in general.