Objectives: To study the feasible application and the optimum conditions for ethanol fermentation using target thermotolerant ethanologenic yeasts isolated from the Mekong Delta, Vietnam; and To study the feasible application and the optimum conditions for fruit wine production using target thermotolerant ethanologenic yeasts isolated from the Mekong Delta, Vietnam. In Vietnam, ethanol is concerned in many different products in terms of fermentation technology including alcoholic drinks and bio-fuel. The raw materials make a competitive in price and food for human needs and animal feed. The diversity of yeast isolates performing high capacities and stability for the controlled processing of alcoholic winemaking and ethanol production from the cheap and available raw materials in region has been studied and achieved. With the aim to pave the way for the application of useful thermotolerant ethanologenic yeasts towards the industrial fermentation technology, the technical feasibilities for the ethanol production and the winemaking by using the selected thermotolerant yeasts have been investigated in the present study. The fermentation capacity of the tested yeasts was monitored and compared at different temperatures (37ºC, 40ºC, 43ºC). Then the selected yeasts were further examined for the optimum fermentation conditions at 37ºC in a factorial design with three factors at three levels, including yeast inoculation level, initial sugar concentration and fermentation time.